| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099906 |
| Chromosome: | chromosome 12 |
| Location: | 6846133 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561101 | ELG42 | Exostosin-like glycosyltransferase 42; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCACCTGGTGAGGGTGTCCGTGTCCGC |
| Internal bar code: | TTGGGGGGGGATTTGGGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 98.9899 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCCGTTGTCTTGATTAC |
| Suggested primer 2: | GTGTTGGTCCTGTCATGTGC |