| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099925 |
| Chromosome: | chromosome 12 |
| Location: | 7334652 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557300 | BLZ10 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2); bZIP transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGCTGGCCGGAGTGCCCATCCAGGGTT |
| Internal bar code: | CATCATCGGAACTAGCCGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 99.7342 |
| LEAP-Seq n confirming: | 4127 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCAACAGCAGCAACAAC |
| Suggested primer 2: | CATCTGCTGCTGGGAGTACA |