| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099938 |
| Chromosome: | scaffold_19 |
| Location: | 61094 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre19.g750447 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTTTTCAAACACGTCGATAACCGCACCA |
| Internal bar code: | CAGATCAGATCGGGTGTCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 98.944 |
| LEAP-Seq n confirming: | 4029 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGTCAAGATGTGCCTC |
| Suggested primer 2: | GCAGCGACTTTGTGTGGTTA |