Insertion junction: LMJ.RY0402.099947_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.099947
Chromosome:chromosome 11
Location:1015987
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467666 LCI13,GT90F43,GT90-43 Low-CO2-inducible 13; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACAAAAGACCGCGCGCCCTCCCGCCCA
Internal bar code:CTGACGTGTTAGGCGCACCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:697
LEAP-Seq percent confirming:99.3214
LEAP-Seq n confirming:1610
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCCATTCCGTCGACAAAC
Suggested primer 2:CATGGTTGAGAGTGTGTGGG