| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100007 |
| Chromosome: | chromosome 17 |
| Location: | 36746 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696350 | (1 of 1) PTHR12454:SF11 - PROTEIN Y57A10A.10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCTGTAATACCTCCGCCGTACATCGGG |
| Internal bar code: | CTCGTTTGAATCCTAGTGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 98.636 |
| LEAP-Seq n confirming: | 3471 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTCGAGGGACAAATGG |
| Suggested primer 2: | TAACGCTCAGTTCGTCATGC |