| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100012 |
| Chromosome: | chromosome 6 |
| Location: | 7402397 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299550 | ZIP6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGTTCAGAACGCGCTGGATAAGAAGAA |
| Internal bar code: | GCCCAAGTATCCGACCCAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 94.7401 |
| LEAP-Seq n confirming: | 15310 |
| LEAP-Seq n nonconfirming: | 850 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGCGTGTGTATCGTTCA |
| Suggested primer 2: | TTTCGTCCCAATGGCTACTC |