| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100014 |
| Chromosome: | chromosome 9 |
| Location: | 1855161 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395750 | (1 of 6) 3.6.3.43 - Peptide-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCCAAGCGGCACGGGAGTAGCACGGC |
| Internal bar code: | ATAGATTGTCGTAAGTTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 99.0724 |
| LEAP-Seq n confirming: | 1068 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACTCCTACAGCCTGCTT |
| Suggested primer 2: | CTACTGGCTGGATGCTGTGA |