| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100015 |
| Chromosome: | chromosome 10 |
| Location: | 2724596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438250 | LAT2 | Latrunculin-B sensitive 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAATGAGGCGGCCACCCATTGGTGCCTG |
| Internal bar code: | CACCGTCCCTTTACCCCCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 99.5531 |
| LEAP-Seq n confirming: | 2005 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAGGTTCTGAACAAGC |
| Suggested primer 2: | GACAACATGTCGCTGATGCT |