| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100024 |
| Chromosome: | chromosome 6 |
| Location: | 7298175 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298650 | HEL30,EIF4A | (1 of 1) PTHR24031//PTHR24031:SF308 - RNA HELICASE // SUBFAMILY NOT NAMED; Eukaryotic initiation factor 4A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAAATGGCTGCAACCGAAGCTGCGCCG |
| Internal bar code: | AACTTTTTCGGTGACGGCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1204 |
| LEAP-Seq percent confirming: | 99.4364 |
| LEAP-Seq n confirming: | 3176 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACTACGACCTGCCCAC |
| Suggested primer 2: | CGTTGCTTCCGATTAACGAT |