| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100028 |
| Chromosome: | chromosome 2 |
| Location: | 9188826 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143587 | (1 of 230) IPR017986 - WD40-repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTGTGGGGAGGTGTTGGTGGGTGCGG |
| Internal bar code: | TTTTAGTCGTAGTTGGAACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 94.5455 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACAGAGAGCTGCACAC |
| Suggested primer 2: | GAGGTGGTGTGTTTGGTGTG |