Insertion junction: LMJ.RY0402.100034_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.100034
Chromosome:chromosome 1
Location:4431248
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g030100 PTK13 Protein tyrosine kinase; (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACTCCATATAAAGGCAACCCATTTCCAG
Internal bar code:ATCCGTTACGACTTTCGGGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1275
LEAP-Seq percent confirming:99.7233
LEAP-Seq n confirming:6847
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGACTTGGTTTTGTCCG
Suggested primer 2:GAGGAACTCAAGCCAACAGC