| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100037 |
| Chromosome: | chromosome 7 |
| Location: | 3859646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338800 | CGL125 | (1 of 4) PF04676 - Protein similar to CwfJ C-terminus 2 (CwfJ_C_2); Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAGTCAGCTGCGACAACTGCTGGGGCT |
| Internal bar code: | CACTAGTTATGTGCGCTCAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 98.8977 |
| LEAP-Seq n confirming: | 1615 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTCAATTGCCTCCAACCC |
| Suggested primer 2: | CGATAAGCCAGCATAGAGGC |