Insertion junction: LMJ.RY0402.100045_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.100045
Chromosome:chromosome 7
Location:1509893
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g324000 NUP160 Nucleoporin 160; (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCATCCCCTAGGTCGGGCATGGCCGCGA
Internal bar code:ATTCGCGTGCGCGGTGGCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:672
LEAP-Seq percent confirming:92.7634
LEAP-Seq n confirming:45891
LEAP-Seq n nonconfirming:3580
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCAAATCCGAATCCTTTC
Suggested primer 2:CTCCGTGTCCCCTACACCTA