| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100056 |
| Chromosome: | chromosome 16 |
| Location: | 1544547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCTGACGCCGCCTGTCTGGCTGCTCAT |
| Internal bar code: | AAGCGATAACAAAGGTGATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 97.0879 |
| LEAP-Seq n confirming: | 1867 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCACAACCTTGCTCATGC |
| Suggested primer 2: | GGCACTTCAGCAAGTTCACA |