| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100058 |
| Chromosome: | chromosome 17 |
| Location: | 4298848 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGGGGAGAGAGGCATGGGGCAGGCCGC |
| Internal bar code: | GACGCCCGGATGGTGCAAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 93.9733 |
| LEAP-Seq n confirming: | 1762 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACAAAAATTTCCGCTCC |
| Suggested primer 2: | GTGAGGAGGAGGCAGAGTTG |