| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100064 |
| Chromosome: | chromosome 17 |
| Location: | 550071 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699700 | Hypothetical protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTGCTCGTACGACCGCATGCACGCCC |
| Internal bar code: | GCGATGGGACGTCACGATTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 98.4143 |
| LEAP-Seq n confirming: | 993 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAAGCTGTTTGGTACGC |
| Suggested primer 2: | CCATGGGATGGTGTGTATCA |