| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100074 |
| Chromosome: | chromosome 16 |
| Location: | 4903902 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685700 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACCAACCAGGACTACTGCAACTACGC |
| Internal bar code: | ATATTCAGACGGGGAGAACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 96.0117 |
| LEAP-Seq n confirming: | 3924 |
| LEAP-Seq n nonconfirming: | 163 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAAGCGGCTTCAAACAC |
| Suggested primer 2: | TACGGTAGGTAGGCACCTGG |