Insertion junction: LMJ.RY0402.100101_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.100101
Chromosome:chromosome 2
Location:3111596
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095058 (1 of 274) IPR020683 - Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAATCCTGACAACCACCACCACCATCTGC
Internal bar code:TTGGGAAGGATCCGTTATTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:919
LEAP-Seq percent confirming:98.8129
LEAP-Seq n confirming:1748
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGGCGAACAAGGTAACAT
Suggested primer 2:CAGCCTCCCTAGACTGTTGC