| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100119 |
| Chromosome: | chromosome 9 |
| Location: | 877074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401551 | BHD1 | 3-beta hydroxysteroid dehydrogenase/isomerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGCCACACTGCTCTTGTACACCTGCAT |
| Internal bar code: | CCTCCTCCCAGCTTCCGAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 81.4558 |
| LEAP-Seq n confirming: | 470 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAACAAATCCTCGCAAC |
| Suggested primer 2: | GTTATATGGCCCAATGCCAC |