| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100126 |
| Chromosome: | chromosome 7 |
| Location: | 1080218 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319800 | (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTGATTGCAATGCGGCGTCGGTTGATA |
| Internal bar code: | GCACGATGAACGCCGGACGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.0093 |
| LEAP-Seq n confirming: | 12492 |
| LEAP-Seq n nonconfirming: | 125 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCTGCGAGAGTCACTG |
| Suggested primer 2: | TCAATCAATGTGTGCGGATT |