Insertion junction: LMJ.RY0402.100129_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.100129
Chromosome:chromosome 6
Location:3761495
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278157 (1 of 1) 3.2.1.143 - Poly(ADP-ribose) glycohydrolase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTCTTATTGTGTCCGACCGCTCAGACAG
Internal bar code:ATTAGTCTGTCCAAACATGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:62
LEAP-Seq percent confirming:97.619
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTCTCACCAGCTCCACCT
Suggested primer 2:ATGTATGTGGGCCATTGCTT