| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100138 |
| Chromosome: | chromosome_2 |
| Location: | 313068 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g075350 | CNK1 | NimA-related protein kinase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGAACTTGCGGACGCAGCACAGGACTGGC |
| Internal bar code: | GACGGCGAAGAATATCGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 391 |
| LEAP-Seq percent confirming: | 99.3625 |
| LEAP-Seq n confirming: | 4832 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAGATGATGACGTTCCG |
| Suggested primer 2: | GGTGCTTTGGATCCTGTTGT |