| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100149 |
| Chromosome: | chromosome 5 |
| Location: | 2697965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236700 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATTGCTTTGCCTTACCTCCAGCGACTT |
| Internal bar code: | TCCCCATTAACTGGGATTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 99.5549 |
| LEAP-Seq n confirming: | 6486 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCTCATTGGGGGTAGT |
| Suggested primer 2: | AGCTGAAACCCGAGAACTCA |