| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100154 |
| Chromosome: | chromosome 1 |
| Location: | 4376214 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029600 | (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCCCCTAACCCCTCACTCACGTGCCG |
| Internal bar code: | AACCGCTGTGCGACACAGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 97.2378 |
| LEAP-Seq n confirming: | 5562 |
| LEAP-Seq n nonconfirming: | 158 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGTACACGCAACGCAAG |
| Suggested primer 2: | CACCCCCTTCCAGTTAGACA |