| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100157 |
| Chromosome: | chromosome 2 |
| Location: | 5863270 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111300 | (1 of 5) PF13391 - HNH endonuclease (HNH_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACCGAACCCGACCCAAACCATCGTTGA |
| Internal bar code: | AATTCTTTCATCGATCAAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 87.3316 |
| LEAP-Seq n confirming: | 3047 |
| LEAP-Seq n nonconfirming: | 442 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCGGTCTACCTGTACTGA |
| Suggested primer 2: | CATGCTACGGAAGAAGGAGC |