| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100174 |
| Chromosome: | chromosome 16 |
| Location: | 1564206 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653601 | MRPL33 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGCATCCTCGTAACAGTCAAACATCAA |
| Internal bar code: | GGACTCCAAAAGATGAGTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 97.8138 |
| LEAP-Seq n confirming: | 6040 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGAGTGCGCTTGTTAG |
| Suggested primer 2: | GTGCAACACCTGTACATCCG |