| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100190 |
| Chromosome: | chromosome 14 |
| Location: | 1468804 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGCGGTAGTCAGCAAAGTAAAAAGTAT |
| Internal bar code: | ATACTCGTATGGAGTCTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.6602 |
| LEAP-Seq n confirming: | 5866 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGGTTGGGCTAGTGAA |
| Suggested primer 2: | CGGCTCTTGCTAAGTCAACC |