| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100199 |
| Chromosome: | chromosome 6 |
| Location: | 8884313 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310950 | FAO7 | (1 of 1) 1.5.8.3 - Sarcosine dehydrogenase; FAD-dependent oxidoreductase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTCGAAGGCTGGAGCCAACCGTCGGCA |
| Internal bar code: | CAACCGCGAGGGAGGTCAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.7234 |
| LEAP-Seq n confirming: | 29201 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGCGGGTACATGCCTAC |
| Suggested primer 2: | ATAGGCAATTTTGGCACGAC |