Insertion junction: LMJ.RY0402.100209_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.100209
Chromosome:chromosome 6
Location:8684140
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g309650 (1 of 1) IPR003200//IPR006626//IPR011050 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like // Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCTCGCCACTGCTCAGCTACAGCTCCG
Internal bar code:GGGCAATCGAAGTGGCGTAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:99.0382
LEAP-Seq n confirming:4325
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAAAAACATCTCCGCAAC
Suggested primer 2:CGAACATGAAGCTACTGGCA