| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100220 |
| Chromosome: | chromosome 10 |
| Location: | 2047975 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432950 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGTGCTGGAGCTGGCCCCAGCAGCAG |
| Internal bar code: | GGCATGTGGCAACTTGCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 96.0817 |
| LEAP-Seq n confirming: | 11672 |
| LEAP-Seq n nonconfirming: | 476 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGGTACTGCCGAATGCT |
| Suggested primer 2: | CTGATCCTCCTGCGTTTGTT |