| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100226 |
| Chromosome: | chromosome 17 |
| Location: | 5849408 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739700 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTGGACCATAAAGCTGGCTCTTCCCAG |
| Internal bar code: | TAAGGTCATTCGACACGGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 89.5082 |
| LEAP-Seq n confirming: | 546 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCGTCCGATTGGTTGTC |
| Suggested primer 2: | TCTAGTCGAGCGTGGGAACT |