Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.100230 |
Chromosome: | chromosome 8 |
Location: | 2562606 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g372500 | (1 of 1) K11373 - elongator complex protein 1 (ELP1, IKI3, IKBKAP) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCCCCTGTACCTTTGTACCTGGCTAC |
Internal bar code: | CTTGCGCCTACCCGGTTTTGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 251 |
LEAP-Seq percent confirming: | 97.8156 |
LEAP-Seq n confirming: | 2239 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACACATCCAACACTCGTCC |
Suggested primer 2: | GTTGTGCCATTTACTGCACG |