| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100248 |
| Chromosome: | chromosome 16 |
| Location: | 7117202 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680230 | SEC61G | SEC61-gamma subunit or ER translocon | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCATGATGACCTGGTTGATGGGCTGCGG |
| Internal bar code: | CTCTCGTCCCGTCTCCCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 98.8842 |
| LEAP-Seq n confirming: | 5672 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTACCACTGTCCCATCGC |
| Suggested primer 2: | AATGTGAGAGCGCATCAGTG |