| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100253 |
| Chromosome: | chromosome 16 |
| Location: | 1147654 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650300 | HTR39,HTR25 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACCAGCGGCTGCGCCACACACCTTGCA |
| Internal bar code: | ACACCCTGGGCTCATTGGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1245 |
| LEAP-Seq percent confirming: | 99.8223 |
| LEAP-Seq n confirming: | 4494 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGAGGACACCAACCTGT |
| Suggested primer 2: | CTCAGCACGCATGTAAGCAT |