| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100254 |
| Chromosome: | chromosome 3 |
| Location: | 7268821 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207351 | (1 of 2) PF10998 - Protein of unknown function (DUF2838) (DUF2838) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGAAGCCCCCAAACTGCCATGTGCCC |
| Internal bar code: | GAGGTGGTTGCAAGACCTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTTTCCGCAACTCACTG |
| Suggested primer 2: | ACGTAGTTGAACAGGGTCCG |