| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100261 |
| Chromosome: | chromosome 7 |
| Location: | 3504645 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335950 | (1 of 2) K10523 - speckle-type POZ protein (SPOP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGCAGCAGGCGACCCGCACACACACAC |
| Internal bar code: | ACCAAATCCCCGAAAAGAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 99.1546 |
| LEAP-Seq n confirming: | 1642 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTTTTGTTTGTGCGTG |
| Suggested primer 2: | ATTTGGGAGATGAAGCATCG |