| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100271 |
| Chromosome: | chromosome 12 |
| Location: | 7829407 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553250 | PFK2 | (1 of 3) K00850 - 6-phosphofructokinase 1 (pfkA, PFK); Phosphofructokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCACGCCACGCCTCCACAGGTTGAGGC |
| Internal bar code: | CATTCAGCGTCGCATCATAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 98.7442 |
| LEAP-Seq n confirming: | 13367 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTACACATCCCTCTGT |
| Suggested primer 2: | ATTCGTGTAACGGCACATCA |