Insertion junction: LMJ.RY0402.100284_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.100284
Chromosome:chromosome 9
Location:6951898
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g410500 PAP11 (1 of 1) IPR002934//IPR023214 - Polymerase, nucleotidyl transferase domain // HAD-like domain; Class-II RNA nucleotidyl transferase 11 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAAACAAACACTACTCCTGCGCATACCC
Internal bar code:GGGTTCATGTATCTGGCGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:200
LEAP-Seq percent confirming:98.5714
LEAP-Seq n confirming:276
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCCTTCTCACCTCCTGC
Suggested primer 2:AAGGTGATGGGGGTTAAAGG