Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.100296 |
Chromosome: | chromosome 4 |
Location: | 1166326 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g215750 | (1 of 1) K02180 - cell cycle arrest protein BUB3 (BUB3) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTTCTCGCACCACGCACCCCTGCTGG |
Internal bar code: | TGCAATGCGCTAAATCGTGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 991 |
LEAP-Seq percent confirming: | 99.5438 |
LEAP-Seq n confirming: | 2400 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCACCTTTCTCTGGAATGT |
Suggested primer 2: | ATCCCAGGTAGCATGTGGAC |