Insertion junction: LMJ.RY0402.100297_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.100297
Chromosome:chromosome 6
Location:2047634
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g264550 CGL81 (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997); Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGAAGCAGCGCGCTACGTTTAAGGCCAG
Internal bar code:CAGTGCACGCGCGTCCCATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:666
LEAP-Seq percent confirming:98.9197
LEAP-Seq n confirming:3388
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCACGTATGCACCAAATC
Suggested primer 2:TGAGCTCGCCAACTACAATG