Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.100297 |
Chromosome: | chromosome 6 |
Location: | 2047634 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g264550 | CGL81 | (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997); Conserved in the Green Lineage | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAGCAGCGCGCTACGTTTAAGGCCAG |
Internal bar code: | CAGTGCACGCGCGTCCCATTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 666 |
LEAP-Seq percent confirming: | 98.9197 |
LEAP-Seq n confirming: | 3388 |
LEAP-Seq n nonconfirming: | 37 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCACGTATGCACCAAATC |
Suggested primer 2: | TGAGCTCGCCAACTACAATG |