| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100302 |
| Chromosome: | chromosome 8 |
| Location: | 4606645 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383300 | Cre10.g429000; (1 of 2) PTHR12668//PTHR12668:SF5 - TRANSMEMBRANE PROTEIN 14, 15 // UPF0136 MEMBRANE PROTEIN YJR085C | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTCGTGCAGCACGCTGATCGCCACAAC |
| Internal bar code: | GCAAGGTGATGGACGCCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 67.6422 |
| LEAP-Seq n confirming: | 2034 |
| LEAP-Seq n nonconfirming: | 973 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGATGCATTTTTGCCTT |
| Suggested primer 2: | GTGTGTGGCTTGGTTGATTG |