| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100316 |
| Chromosome: | chromosome 13 |
| Location: | 4894495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605850 | OPR127 | OctotricoPeptide Repeat protein 127 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTTCCAGTAAACTCCACAAATTACATGG |
| Internal bar code: | CTGCTCCTCCAGCTTTGCGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 139 |
| LEAP-Seq percent confirming: | 93.5655 |
| LEAP-Seq n confirming: | 1585 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGACAATTCATCCAGGC |
| Suggested primer 2: | CCTAGGGGATTCACGACGTA |