| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100324 |
| Chromosome: | chromosome 9 |
| Location: | 2394059 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391753 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAAGTTGATCGCTGAAGCCGTGCATGC |
| Internal bar code: | TCGCTTTAGCGAAGGCGTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.3419 |
| LEAP-Seq n confirming: | 3774 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATACAACGGAACGCACA |
| Suggested primer 2: | CTGTACGTGTCATCGGCATC |