| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100340 |
| Chromosome: | chromosome 9 |
| Location: | 6551032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407900 | RRM8 | (1 of 1) PTHR11061//PTHR11061:SF15 - RNA M5U METHYLTRANSFERASE FAMILY // SUBFAMILY NOT NAMED; Putative rRNA (guanine-N2-)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGAGAACTTGAATCTCCACGGCTCCACC |
| Internal bar code: | TTTCTCGCCGATTAGCTTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1042 |
| LEAP-Seq percent confirming: | 90.2795 |
| LEAP-Seq n confirming: | 743 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGTTGCTTGATGTTGGT |
| Suggested primer 2: | AGCCGAAGTCGAAATGCTAA |