| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100375 |
| Chromosome: | chromosome 6 |
| Location: | 7517759 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300550 | TIC100 | (1 of 14) PF02493 - MORN repeat (MORN); 100 kDa subunit of translocon at the inner membrane of chloroplasts | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACGCCGCCTCCGGCTGCCCCTAGCTGC |
| Internal bar code: | TGATCACACGGTCGGCAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 80.3279 |
| LEAP-Seq n confirming: | 343 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATCTAGGTGGCAGCAGG |
| Suggested primer 2: | CCATGTCCTCTGTCAACCCT |