| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100428 |
| Chromosome: | chromosome 12 |
| Location: | 5802859 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAATGCTAGAGCTGGCCAAAGTGGCAAC |
| Internal bar code: | GCGGTTACTCGATAGCGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 80.7899 |
| LEAP-Seq n confirming: | 450 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCCTCCTAAGACCACG |
| Suggested primer 2: | CAGTGAGTCCTAGCCAAGCC |