Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.100443 |
Chromosome: | chromosome 12 |
Location: | 3493245 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g501717 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAACGCGTACGGAATTCCAACCGGAACC |
Internal bar code: | CAGTCAACCGTGGCGTGCGCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 571 |
LEAP-Seq percent confirming: | 96.648 |
LEAP-Seq n confirming: | 173 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATCGCCTTTCACTGCTACA |
Suggested primer 2: | CACCGACTGTACTGACCCCT |