Insertion junction: LMJ.RY0402.100443_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.100443
Chromosome:chromosome 12
Location:3493245
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g501717 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTAACGCGTACGGAATTCCAACCGGAACC
Internal bar code:CAGTCAACCGTGGCGTGCGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:96.648
LEAP-Seq n confirming:173
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CATCGCCTTTCACTGCTACA
Suggested primer 2:CACCGACTGTACTGACCCCT