| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100467 |
| Chromosome: | chromosome 6 |
| Location: | 2491030 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATCGTGAGCTCGGCTGGGCCTGCCTGC |
| Internal bar code: | CGGTCCCCGAGATAGGGGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 96.8172 |
| LEAP-Seq n confirming: | 2251 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTCACTTGGGTTCTTT |
| Suggested primer 2: | GGAAGCAGATGCACACAGAA |