Insertion junction: LMJ.RY0402.100469_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.100469
Chromosome:chromosome 17
Location:92492
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGACCAAAGGCATCCGAGTCGCACTCGTG
Internal bar code:AGAGACCGCAGCGGGGTTACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:731
LEAP-Seq percent confirming:99.4508
LEAP-Seq n confirming:2535
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCAAAGTGGTGTCAATG
Suggested primer 2:GTGGTATCTGGGTCAACGCT