Insertion junction: LMJ.RY0402.100486_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.100486
Chromosome:chromosome 15
Location:1255107
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATATGGTGCCATCTGCGCCAGGAACAGCC
Internal bar code:TCAATCCGTCCACTCCGATAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:85.431
LEAP-Seq n confirming:4609
LEAP-Seq n nonconfirming:786
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCTGTGTAGGCTTTGCC
Suggested primer 2:TGGAATCTAGCACTGCATCG